Chromatin Immunoprecipitation:Article Title: Harnessing 3D collagen hydrogel-directed conversion of human GMSCs into SCP-like cells to generate functionalized nerve conduits
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used For IF: mouse anti-p75 (5 μg/mL, Sigma, St. Louis, Missouri, USA), rabbit anti-SOX-9 (5μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-HES1 (5μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), mouse anti-SOX10 (5μg/mL, R & D System, Minneapolis, Minnesota, USA), rabbit anti-S-100beta (5μg/mL, Boster Biological Tech, Pleasanton, California), rabbit antiNotch3 (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), rabbit anti-BDNF (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), rabbit anti-GDNF (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), rabbit anti-NGF (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), mouse anti-vinculin (5 μg/mL, Sigma, St. Louis, Missouri, USA), TRITC-phalloidin (0.5 μg/mL, Sigma, St. Louis, Missouri, USA), mouse anti-neurofilament (5μg/mL, BioLegend, San Diego, California, USA), goat anti-rabbit IgG, Alexa Fluor 488, goat anti-rabbit IgG, Alexa Fluor 594, goat anti-mouse IgG, Alexa Fluor 488, goat anti-mouse IgG, Alexa Fluor 594, or normal anti-rabbit, normal anti-mouse IgG, Alexa Fluor 488 , Alexa Fluor 594 (2.5 μg/mL, BioLegend, San Diego, California, USA). .. For Western blot: rabbit anti-p75 (1μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-HES1 (1μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-GAPDH (1 μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-Notch3 (1μg/mL, Abcam, Cambridge, Massachusetts, USA).
Flow Cytometry:Article Title: Harnessing 3D collagen hydrogel-directed conversion of human GMSCs into SCP-like cells to generate functionalized nerve conduits
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used For IF: mouse anti-p75 (5 μg/mL, Sigma, St. Louis, Missouri, USA), rabbit anti-SOX-9 (5μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-HES1 (5μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), mouse anti-SOX10 (5μg/mL, R & D System, Minneapolis, Minnesota, USA), rabbit anti-S-100beta (5μg/mL, Boster Biological Tech, Pleasanton, California), rabbit antiNotch3 (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), rabbit anti-BDNF (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), rabbit anti-GDNF (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), rabbit anti-NGF (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), mouse anti-vinculin (5 μg/mL, Sigma, St. Louis, Missouri, USA), TRITC-phalloidin (0.5 μg/mL, Sigma, St. Louis, Missouri, USA), mouse anti-neurofilament (5μg/mL, BioLegend, San Diego, California, USA), goat anti-rabbit IgG, Alexa Fluor 488, goat anti-rabbit IgG, Alexa Fluor 594, goat anti-mouse IgG, Alexa Fluor 488, goat anti-mouse IgG, Alexa Fluor 594, or normal anti-rabbit, normal anti-mouse IgG, Alexa Fluor 488 , Alexa Fluor 594 (2.5 μg/mL, BioLegend, San Diego, California, USA). .. For Western blot: rabbit anti-p75 (1μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-HES1 (1μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-GAPDH (1 μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-Notch3 (1μg/mL, Abcam, Cambridge, Massachusetts, USA).
Magnetic Resonance Imaging:Article Title: Harnessing 3D collagen hydrogel-directed conversion of human GMSCs into SCP-like cells to generate functionalized nerve conduits
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used For IF: mouse anti-p75 (5 μg/mL, Sigma, St. Louis, Missouri, USA), rabbit anti-SOX-9 (5μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-HES1 (5μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), mouse anti-SOX10 (5μg/mL, R & D System, Minneapolis, Minnesota, USA), rabbit anti-S-100beta (5μg/mL, Boster Biological Tech, Pleasanton, California), rabbit antiNotch3 (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), rabbit anti-BDNF (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), rabbit anti-GDNF (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), rabbit anti-NGF (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), mouse anti-vinculin (5 μg/mL, Sigma, St. Louis, Missouri, USA), TRITC-phalloidin (0.5 μg/mL, Sigma, St. Louis, Missouri, USA), mouse anti-neurofilament (5μg/mL, BioLegend, San Diego, California, USA), goat anti-rabbit IgG, Alexa Fluor 488, goat anti-rabbit IgG, Alexa Fluor 594, goat anti-mouse IgG, Alexa Fluor 488, goat anti-mouse IgG, Alexa Fluor 594, or normal anti-rabbit, normal anti-mouse IgG, Alexa Fluor 488 , Alexa Fluor 594 (2.5 μg/mL, BioLegend, San Diego, California, USA). .. For Western blot: rabbit anti-p75 (1μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-HES1 (1μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-GAPDH (1 μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-Notch3 (1μg/mL, Abcam, Cambridge, Massachusetts, USA).
Blocking Assay:Article Title: Whole-genome analysis of noncoding genetic variations identifies multiscale regulatory element perturbations associated with Hirschsprung disease
Article Snippet: .. Fixed cells were blocked with blocking solution (1% BSA, 0.1% Triton X-100 in PBS) at room temperature for 1 h. The blocked cells were then incubated with primary antibodies (mouse anti-SOX10 [1:500, R&D Systems MAB2864], rabbit anti-TUJ1 [1:1000, Abcam ab18207] and mouse anti-HU [1:1000, Thermo Fisher Scientific A-21271]) at 4°C overnight. .. After washing, the sections were incubated with respective secondary antibodies conjugated with Alexa Fluor 488/594 (Thermo Fisher Scientific 1:500) at room temperature for 2 h. The stained cells were mounted with ProLong Diamond Antifade Mountant with DAPI (Thermo Fisher Scientific).
Incubation:Article Title: Whole-genome analysis of noncoding genetic variations identifies multiscale regulatory element perturbations associated with Hirschsprung disease
Article Snippet: .. Fixed cells were blocked with blocking solution (1% BSA, 0.1% Triton X-100 in PBS) at room temperature for 1 h. The blocked cells were then incubated with primary antibodies (mouse anti-SOX10 [1:500, R&D Systems MAB2864], rabbit anti-TUJ1 [1:1000, Abcam ab18207] and mouse anti-HU [1:1000, Thermo Fisher Scientific A-21271]) at 4°C overnight. .. After washing, the sections were incubated with respective secondary antibodies conjugated with Alexa Fluor 488/594 (Thermo Fisher Scientific 1:500) at room temperature for 2 h. The stained cells were mounted with ProLong Diamond Antifade Mountant with DAPI (Thermo Fisher Scientific).
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